Illumina

Conventional molecular microbiology methods have limited capabilities to detect and profile many of the causes of lower respiratory tract infections. As antimicrobial resistance (AMR) is increasing, early and accurate pathogen identification and characterization have become even more crucial recently. With next-generation sequencing (NGS), a wide range of common and rare respiratory pathogens can be detected rapidly in a single test, and AMR characterization can be significantly improved for surveillance applications, in both the healthcare and the environmental sectors.

The new Illumina Respiratory Pathogen ID and AMR Panel (RPIP) is a research use only assay that provides a precision metagenomics solution with high analytical sensitivity for respiratory pathogen identification, and capability for extensive AMR detection. This target enrichment assay identifies and profiles almost 300 species of bacteria, viruses, and fungi, that can cause lower respiratory tract infections, and detects more than 2000 antimicrobial resistance gene markers within a 24-hour workflow. RPIP is compatible with MiniSeq, MiSeq, and NextSeq sequencing platforms.

Robert Schlaberg, VP and Distinguished Scientist in Infectious Disease at Illumina
“Challenges and Advances in Respiratory Pathogen Identification and Surveillance” Robert Schlaberg gives an introduction to respiratory detection and the new Illumina Respiratory Pathogen ID/AMR Panel (RPIP).

In particular, this session is divided in three parts:

  • An introduction to Respiratory Pathogen Identification, Antimicrobial Resistance and Surveillance;
  • An overview of the Respiratory Pathogen ID and AMR Panel (RPIP);
  • A brief summary of analytical performance data.
Dr. Sébastien Boutin, Microbiologist at the Clinic University of Schleswig-Holstein, Clinic for Infectious Diseases and Microbiology, Lübeck, Germany
“Bringing Precision Metagenomics to comprehensive respiratory testing”

Dr. Sébastien Boutin presents an evaluation study conducted in his lab, aiming to assess the efficiency of the Respiratory Pathogen ID/AMR Enrichment Panel Kit (RPIP) compared to microbiological culture methods and PCR, in patients with suspected pneumoniae and tuberculosis.

The overall results demonstrates that RPIP panel is a good alternative as it detects more easily the airways commensals, the slow growers, anaerobes and streptococci.

     

    M-EMEA-00713